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논문분류	춘계학술대회 초록집
제목	The effects of dietary salt on intrarenal immune cells and endothelial cells
저자	Kyungho Lee
출판정보	2023; 2023(1):
키워드
초록	Objectives: Kidney immune cells and endothelial cells play important roles in kidney ischemia-reperfusion injury. We aimed to investigate the effect of dietary salt intake on kidney immune cells and endothelial cells using experimental models and allograft biopsy specimens. Methods: C57BL/6 mice were fed with normal diet, low-salt diet, or high-salt diet, and kidneys were collected after 6 weeks of allocated diets. Kidney sections were stained for CD45. Glycosaminoglycan (GAG), a major sodium buffer, concentration was measured using exsanguinated whole kidney tissue. Kidney lymphocytes were isolated and analyzed with flow cytometry. Human renal allograft tissue obtained on 2 weeks after kidney transplantation was stained with CD45 and CD31 and analyzed according to donors’ 24h urine Na levels. Induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) from healthy individuals and end-stage kidney disease patients were cultured in different Na concentrations and the degree of proliferation was measured. Results: High-salt diet group showed higher intrarenal leukocyte infiltration (CD45+) compared to those with normal diet (1.9±0.3% vs 0.9±0.1%, P=0.043). GAG concentration was lower in high-salt diet group than low-salt group (41.0±3.1 vs 48.3±1.3ng/mg, P=0.004). Low-salt and high-salt diets increased effector-memory CD4+ T cells (normal 44.2±2.6%; low-salt 57.1±3.5%, P=0.020; high-salt 62.1±2.5% of CD4+ T cells, P=0.002) and mature B cells (70.0±1.5%; 79.1±1.4%, P=0.001; 76.7±1.6% of CD19+ cells, P=0.015) in steady-state kidneys. Renal allografts from donors under low-salt diet showed reduced leukocyte infiltration (CD45+, 0.3±0.1% vs 0.9±0.3%, P=0.019) and higher capillary density (CD31+, 29.0±2.2% vs 16.1±1.8%, P=0.009) compared to those from donors under high-salt diet. iPSC-ECs with higher sodium concentrations exhibited reduced proliferation rates after hypoxia followed by reoxygenation. Conclusions: Dietary salt induces changes in kidney immune cell numbers and compositions as well as endothelial cells. Reconstitution of renal microenvironment by modification of dietary salt intake could be a promising strategy to improve kidney transplantation outcomes.
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