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논문분류	춘계학술대회 초록집
제목	Integrin, fibronectin, and upar in podocyte-glomerular basement membrane interaction under fluid shear stress
저자	Jae Seok Kim
출판정보	2023; 2023(1):
키워드
초록	Objectives: Interaction of podocyte with glomerular basement membrane (GBM) determines podocyte stability. On the other hand, the podocyte-GBM interaction could be also changed depending on podocyte’s conditions. We hypothesized that the podocyte-GBM interaction would be changed to withstand stress conditions such as fluid shear stress (FSS). Here, we aim to explore the change of specific integrin (Itg αVβ3), fibronectin, and uPAR in the interaction of podocyte and GBM under FSS. Methods: We used conditionally immortalized human podocyte cells which were differentiated only under specific (non-permissive) temperature (37°C) for cell experiment. We exposed the podocytes to 200 rpm of FSS. Then, we documented the protein and mRNA expressions of Itg β3, fibronectin, and uPAR under FSS. Results: Protein expression of Itg β3 did not change under FSS, while activated Itg β3 increased from early phase (30 min after exposure to FSS) (Figure 1). Fibronectin gradually increased under FSS, while uPAR had increasing trend but not consistent (Figure 2). mRNA expressions of Itg β3, fibronectin, and uPAR did not change under FSS. Conclusions: This study demonstrates that Itg αVβ3 is activated under FSS. Furthermore, activated Itg αVβ3, fibronectin, and possibly uPAR increase under FSS. The results suggest that the podocyte-GBM interaction is dynamic according to podocyte conditions, and plays a specific role to maintain the podocyte’s stability under stress conditions.
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