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논문분류	춘계학술대회 초록집
제목	Characteristics of puromycin-induced kidney injury following regeneration in adult zebrafish model
저자	Hye Jin Park
출판정보	2023; 2023(1):
키워드
초록	Objectives: Puromycin has been known to develop glomerular injury of the kidney leading to proteinuria. However, the pathogenesis for acute kidney injury following regeneration after puromycin administration remains uncertain in animal model. In this study, we examined characteristics of kidney injury following regeneration by puromycin in adult zebrafish model. Methods: We injected 100ug of puromycin via intraperitoneal injection into wild-type zebrafish, which were sacrificed at 1, 3, 5, 7, and 14 dpi. Then, the morphological, functional and molecular changes were examined in the kidney of adult zebrafish. Results: Compared with controls, puromycin-treated zebrafish presented more rapid clearance of 40 kDa rhodamine dextran, which reappeared at 14 dpi during the recovery. By H&E staining, morphological change of the kidney was identified immediately after puromycin injection from 1 dpi to 7 dpi and recovered at 14 dpi. In situ hybridization of lhx1a, as a renal progenitor marker, revealed an increased production during the recovery of kidney injury. There were a significant increase of NFkB, TNFa, Nampt, Sirt1, FOXO1, and p-ERK production from 1 dpi to 5 dpi and then decrease until 14 dpi, and pax2 and wt1b were also increased during nephron injury and regeneration. However, TGFb1 and smad5 production did not show any difference by puromycin treatment. Conclusions: From these results, puromycin-induced injury in adult zebrafish kidney provides potential as a tool for the evaluation of mechanism for nephron injury following regeneration.
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