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논문분류	춘계학술대회 초록집
제목	Fabrication, characterization and anti-fibrotic effect of surface engineered nano-formulation of revesterol against peritoneal fibrosis via alteration of TGF-β1
저자	D Chauhan
출판정보	2023; 2023(1):
키워드
초록	Objectives: We fabricate the nano-formulation of Revesterol (RT-PLGA-NP) and scrutinized against the peritoneal fibrosis in rats and explore the underlying mechanism. Methods: The RT-loaded PLGA nanoparticles (RT-PLGA-NP) were prepared using the emulsion solvent technique and were developed for an economical dosing system. The potential of the CT-PLGA-NP to decrease TGF-β signaling was evaluated through docking analysis. Intraperitoneal administration of 0.1% Chlorhexidine gluconate was used to induce peritoneal fibrosis (PF) in rats. Both inflammatory and biochemical parameters were evaluated. The quantitative RT-PCR was carried out to estimate the levels of inflammation and fibrosis-associated factors. We also detected the Transforming growth factor beta 1 (TGF-β1) in peritoneal fluid by ELISA. Results: The RT-PLGA-NP exhibited the desired properties of nanoparticles, featuring a spherical, smooth appearance and a narrow size distribution. RT-PLGA-NP showed the remarkably reduction the thickness of peritoneal membrane and also myofibroblasts expression with avoidance of loss filtration of peritoneal membrane. RT-PLGA-NP showed the alteration in the antioxidant parameters such as malonaldehyde (38.6%), superoxide dismutase (42.4%), glutathione (36.7%), catalase (52.3%); inflammatory cytokines viz., tumor necrosis factor-α (46.5%), interleukin-1β (45.6%), interleukin-6 (34.5%). RT-PLGA-NP also suppressed the level of TGF-β1 in the peritoneal fluid. Conclusions: The result suggest that the RT-PLGA-NP exhibited the renal protective effect against the peritoneal fibrosis via alteration of TGFβ1.
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