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논문분류	춘계학술대회 초록집
제목	New Strategies for Improving Kidney dECM Recellularization Efficiency
저자	BOYUN KIM
출판정보	2024; 2024(1):
키워드
초록	Objectives: The utilization of decellularized extracellular matrix (dECM) from kidneys has emerged as a pivotal strategy in renal tissue engineering, offering a natural scaffold for regenerative applications and as a bioartificial organ for transplantation. This study explores the synergistic impact of albumin coating on kidney dECM to enhance the recellularization processes. It introduces a novel approach employing albumin and a rotating culture system to elevate recellularization efficiency and mitigate thrombosis. Methods: Porcine kidney decellularization was optimized through whole organ perfusion or slice immersion. Subsequently, the dECM was coated with an albumin solution and cultured in a rotating system. Cell proliferation was quantified using ELISA, while cell penetration into the tissue was evaluated through fluorescence staining. Results: The application of albumin coating and a rotation culture system significantly improved the recellularization efficiency of kidney dECM. The albumin-coated dECM exhibited notable enhancements in cell attachment and spreading, fostering an advantageous microenvironment for renal cell repopulation. Furthermore, albumin coating demonstrated efficacy in preventing blood clot formation during perfusion post-recellularization. The rotation culture process contributed to an increased cell proliferation rate by enhancing cell penetration into the tissues. Conclusions: Albumin coating of kidney dECM scaffolds emerged as a powerful strategy to augment recellularization by facilitating cell adhesion and proliferation. Notably, albumin coating exhibited efficacy in preventing thrombus formation. These findings advance the development of improved dECM-based scaffolds, offering enhanced recellularization potential and reduced thrombosis risk. Ultimately, this research contributes to the progress of kidney regenerative medicine and transplantation.
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