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논문분류	춘계학술대회 초록집
제목	Molecular pathology patterns in podocytopathy: a pilot study
저자	Darya Narygina
출판정보	2024; 2024(1):
키워드
초록	Objectives: This pilot study was focused to evaluate WT-1, β-catenin, desmin and vimentin glomerular expression patterns in early primary focal segmental glomerulosclerosis (FSGS). Methods: We reviewed the histopathological and clinical data of patients with biopsy proven FSGS (N=14, experimental group), IgA nephropathy (N=12, positive control) without AKI, infectious diseases, heart failure, respiratory insufficiency, cancer pathology and immunosuppressive therapy. As a negative control, kidney samples without glomerulopathies (GP) taken from surgery for renal cancer were tested (N=12). Glomerulosclerosis was assessed as percentage of all glomeruli. Mesangial lesions were measured semi-quantitatively in scores (0-3). Glomerular WT-1, β-catenin, desmin, and vimentin expression was assessed by IHC-P with quantification of IHC-stained to glomerular area (QuPath, Orbit Image Analysis). Molecules' co-expression was analyzed by IF-p and confocal microscopy (Center for Collective Use, Pavlov Institute of Physiology). Results: The comparison of clinical and histopathological data between GP groups shown in Table 1. An immunomorphological study showed lower WT-1 expression in both GP groups compared to the negative control (Figure 1a, d). B-catenin expression was significantly higher in IgA and did not differ between FSGS and negative control (Figure 1b, d). In FSGS, β-catenin expressed lesser vs. IgAN, and co-expressed with desmin occasionaly (Figure 1f). A trend towards to increase in the desmin+ glomerular area was also detected, however, no significant differences between IG groups were found; as well as for vimentin (Figure 1c, d). In IgAN, glomerular WT-1 and β-catenin co-expression was prevalent (Figure 1e). In FSGS, glomerular cells were predominantly desmin+ and negative to WT-1/ β-catenin; WT-1+cells mainly co-expressed desmin (Figure 1f). Conclusions: A decrease in WT-1 is an early feature of both FSGS and IgA glomerulopathies. In contrast to IgA, which is characterized by an increase in beta-catenin expression, desmin expression may be an early pathognomonic feature of FSGS.
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