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제목	Flavonoid-Rich Clitoria Ternatea Aqueous Extract Ameliorates Kidney Morphologic Damage Through Inhibition of HMG-CoA Reductase Pathway on Rats With High-Fat Diet: in Vivo With Molecular Docking Study
저자	I Gede Aswin Parisya Sasmana
출판정보	2025; 2025(1):
키워드	Clitoria ternatea, flavonoid, high-fat diet, HMG-CoA reductase inhibitor, kidney damage
초록	Obesity and dyslipidemia incidences have increased in the general population in line with the increase in fat consumption. These conditions contribute to systemic organ disruption, including the kidney, as a result of lipid imbalance and systemic inflammation that occur. Clitoria ternatea (CT) flower is found to have rich flavonoid content and potentially reduce obesity and dyslipidemia-related complications through inhibition of HMG-CoA reductase, the precursor enzyme for cholesterol synthesis. This study aims to determine the potency of CT aqueous extract in ameliorating kidney damage in rats with high-fat diet. An experimental study was conducted involving 25 Wistar rats which were divided into a normal group (ND), a high-fat diet (HFD) group, and three of the treatment groups with CT extract (HFD+CT250; HFD+CT500; HFD+CT750). We harvested the kidney then processed histological preparation and valued it by using ImageJ software. Phytochemical and molecular docking analysis was also performed. CT extract was found to have 1771,39 mg/100g flavonoid content. Macroscopic examination showed a significant reduction of kidney weight in the HFD+CT750 groups compared to the HFD group (1.65±0.30 vs 2.26±0.47 g; p= 0.02). The amelioration of kidney morphology was represented by a significant reduction in Bowman’s space of the HFD+CT500 group compared to the HDF group (0.53±0.39 vs 3.78±1.03 μm; p= 0.018). CT extract is also able to inhibit the glomerular tuft area enlargement with the similar area compared to the ND group (31.28±8.51 vs 20.0±4.26 μm; p= 0.3). Docking analysis shows that delphinidin, a type of flavonoid on CT extract, has the potency to inhibit HMG-CoA reductase with lower bonding energy compared to simvastatin (-8.0 vs -7.0 kcal/mol) thus showing the potency to inhibit dyslipidemia-related condition. Clitoria ternatea extract has rich flavonoid content with potentially ameliorates kidney morphology damage through inhibition of HMG-CoA reductase in rats with high-fat diet.
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