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논문분류	춘계학술대회 초록집
제목	Proteomic Analysis Identifies RhoA as a Novel Therapeutic Target for Peritoneal Fibrosis
저자	Seongmin Cheon
출판정보	2025; 2025(1):
키워드	Peritoneal dialysis, Peritoneal fibrosis, Proteomics, RhoA, RhoA inhibitor
초록	Peritoneal dialysis (PD) is an essential treatment for patients with chronic kidney disease (CKD). However, peritoneal fibrosis (PF), a common complication of PD, significantly impairs its efficacy and increases the risk of peritonitis, cardiovascular diseases, and the progression of CKD. Here, we analyzed the proteome of human peritoneal mesothelial cells (hPMCs) from CKD patients and conducted drug connectivity analyses to identify key biomolecules linked to PF, particularly focusing on the RhoA protein. hPMCs were isolated from PD effluent and were treated with 2 ng/mL rTGF-β for 48 hours to induce fibrosis. TMT tag-based quantitative proteomic analysis was conducted to investigate the global proteome profile of hPMCs. Identified differentially expressed proteins (DEPs) were subjected to pathway analysis using the KEGG Database and drug connectivity analysis using the Proteomic Atlas for Small Molecule Perturbagens Database to identify potential drug targets. A total of 8,961 proteins were quantified in hPMCs. Treatment with rTGF-β induced a significant increase in the expression of key extracellular matrix proteins, including FN1, COL1A1, and TAGLN. DEPs were further selected based on their increased expression in response to rTGF-β treatment for drug connectivity analysis. Potential therapeutic candidates were identified, including inhibitors of RhoA, PDGFR, and tyrosine kinase, which may counteract the gene expression patterns associated with PF. Notably, RhoA, an important GTPase that regulates myofibroblast differentiation, was found to be highly expressed in hPMCs, highlighting its potential as a therapeutic target for PF. These findings suggest that targeting RHOA, along with other identified inhibitors, could offer promising therapeutic strategies for mitigating PF.
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