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논문분류
제목	An Evidence of Transdifferentiation of Principal Cells into Intercalated Cells in Hypokalemic AQP2-cre;ROSA Mouse Kidney
저자	Wan-Young Kim, Sun-Ah Nam, Yu-Mi Kim, Jin Kim
출판정보	2013; 2013(1):
키워드	형질전환 생쥐, 산-염기 균형, 저칼륨혈증/ Transgenic mouse, Acid-base balance, Hypokalemia
초록	In K+-depleted animals, there was a reversible increase in number of intercalated cells (ICs) in the inner stripe part of outer medullary collecting duct (OMCD), but the cell proliferation had been mainly detected in the principal cells (PCs). A suggested mechanism to explain the increase of ICs in K+-depleted animal was a direct or indirect convertsion of PCs to ICs. The purpose of this study was to propose the possible transdifferentiation of PCs to ICs using a kidney of hypokalemic AQP2-cre;ROSA mouse. Adult male transgenic mice (AQP2-cre;ROSA) were used in the study and the experimental group was fed with K+-depleted diet for two weeks. Each ICs and PCs were immunolabeled with specific markers for H+-ATPase and AQP2, respectively. As a result, in the control group, ROSA-positive signal was mainly evidenced in PCs (35.4% of H+-ATPase-negative/AQP2-positive PCs), but a few ICs showed ROSApositive signal (5.5% of H+-ATPase-positive/AQP2-negative ICs) even in the normal kidney. In hypokalemic kidney, the proportion of ROSA-positive cells in ICs was significantly increased to 16.2% but the proportion of ROSA-positive cells in PCs (37.0% of H+-ATPase-negative/AQP2-positive PCs) was comparable to the control group. In conclusion, we propose a mechanism of transdifferentiation of the ICs from AQP2-ROSA-positive PCs in OMCD, causing the increase in number and proportion of ICs at least in hypokalemic mice.
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