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논문분류	춘계학술대회 초록집
제목	Leptin-induced Epithelial-mesenchymal Transition in Human Peritoneal Mesothelial Cell
저자	San Ok Kim, Seok Hui Kang, Yun Mi Suk, Kyu Hyang Cho,Jong Won Park, Kyung Woo Yoon, Jun Young Do
출판정보	2013; 2013(1):
키워드
초록	Epithelial-mesenchymal transition (EMT) is an important problem in the maintenance of peritoneal dialysis. Some studies have reported that higher levels of leptin is a cause of tumor progression and involved in EMT. We investigated the effects of leptin on human peritoneal mesothelial cells (HPMCs). In vitro, HPMCs were exposed to median (control), media+TGF-β, media+TGF-β+leptin (50 ng/mL), and media+TGF-β+leptin (100 ng/mL). Cell morphology was analyzed under an inverted phase-contrast microscope. Protein expression of EMT markers such as α-SMA and Ecadherin were evaluated by western blot assay. The cobblestone- like appearance of mesothelial cells were converted to a fibroblast-like morphology after treatment with TGF-β and/or leptin. E-cadherin expression was significantly decreased in HPMCs exposed to TGF-β. E-cadherin expression in HPMCs treated with TGF-β and leptin was lower than in those exposed to only TGF-β. The trend for α-SMA expression was reverse to that in the E-cadherin. This study demonstrated that leptin-induced EMT may be applicable to HPMCs.
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