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논문분류	춘계학술대회 초록집
제목	Alpha-lipoic Acid Attenuates Lipopolisaccharide-induced Kidney Injury
저자	Sang Heon Suh1, Ko Eun Lee1,2, Ok Kim2, Chang Seong Kim1, Joon Seok Choi1,Eun Hui Bae1, Seong Kwon Ma1, Jong Un Lee2, Soo Wan Kim1
출판정보	2013; 2013(1):
키워드	Alpha-lipoic acid, Apoptosis, Inflammation, Kidney, Lipopolysaccharide
초록	Background: Kidney is one of the major target organs in sepsis, while effective prevention of septic acute kidney injury (AKI) has not yet been established. Alpha-lipoic acid (ALA) has been known to exert beneficial effects against lipopolysaccharide (LPS)-induced damages in various organs, such as heart, lung, and liver, but not in kidney. We investigated the protective effect of ALA on LPS-induced kidney injury. Methods: Two groups of rats were treated with LPS (20 mg/kg, i.p.), one of which being cotreated with ALA (50 mg/kg). The control group was treated with vehicle only. Human renal proximal tubular epithelial cells (HK-2 cells) were cultured with LPS (10 μg/ml) in the absence or presence of ALA (210 μg/ml) for 3 hours prior to LPS treatment. Results: Plasma creatinine level was increased in LPS treated rats, which was attenuated by ALA co-treatment. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive cells increased in the kidneys of LPS treated rats compared with controls, which was counteracted by ALA treatment. 4',6-diamidino-2-phenylindole (DAPI) staining also revealed nuclear fragmentation, condensation and shrinkage and heavy staining in HK-2 cells treated with LPS, which was prevented by ALA. LPS treatment induced cleaved caspase-3, inducible nitric oxide synthase (iNOS), aquaporin (AQP) 2 expression, which were counteracted by ALA in immunoblotting of reanl cortex and outer stripe of outer medulla. Increased protein expression of phospho-extracellular signal-regulated kinases 1/2 and c-Jun N-terminal kinases by LPS treatment was attenuated by ALA pretreatment, while p38 was not affected by either LPS or ALA treatment in HK-2 cells. Immunohistochemical staining demonstrated the expression of cyclooxygenase-2 and iNOS was enhanced, while that of AQP 1 was decreased in the kidneys from LPS rats, which was attenuated by ALA cotreatment Conclusion: ALA treatment attenuates LPS-induced kidney injury by suppression of apoptosis, inflammation, and renal tubular dysfunction.
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