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제목	Chymase Inhibition Ameliorates Apoptosis and Fibrosis in Hypoxic Injury of Renal Proximal Tubular Epithelial Cells
저자	Seong Kwon Ma1, Soo Yeon Joo1, Gi Sun Baek1, Hoon-In Choi1,Jung Sun Park1, Eun Hui Bae1, Jong Un Lee2, Soo Wan Kim1
출판정보	2014; 2014(1):
키워드	Chymase, Apoptosis, Fibrosis
초록	Angiotensin II (Ang II) plays a crucial role in the pathogenesis of kidney diseases. Chymase is an alternative pathway for angiotensin converting enzyme (ACE) in Ang II formation. Previous studies demonstrated that chymase plays an important role in tissue remodeling through the activation of transforming growth factor (TGF)-β1. However, the pathophysiological roles of chymase in the pathogenesis of kidney diseases have not yet been identified. We investigated the effects of chymase inhibition on the apoptosis and fibrosis induced by hypoxia in human renal proximal tubular epithelial (HK-2) cells. HK-2 cells were cultured in hypoxic chamber in the absence or presence of chymostatin, a chymase inhibitor. In HK-2 cells, hypoxia increased the expression of chymase. The protein expression of Bax/Bcl-2, cleaved caspase-3 and TGF-β1 was also increased. Hypoxic treatment resulted in decreased cell viability. The pretreatment of chymostatin ameliorated the decreased cell viability by hypoxia. Hypoxia-induced increase in the expression of Bax/Bcl-2, cleaved caspase-3 and TGF-β1 was also counteracted by the pretreatment of chymostatin. These findings suggest that chymase inhibition may play a protective role in the hypoxic renal tubular injury through the inhibition of pro-apoptotic and pro-fibrotic proteins.
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