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논문분류	춘계학술대회 초록집
제목	The Effects of Endothelial Microparticle on the Neointimal Hyperplasia Formation and Vascular SMC Proliferation
저자	Jung-Hwa Ryu1, Heejung Park2, Shina Lee1, Hee Sung Ko1, Dong-Ryeol Ryu1,Duk-Hee Kang1, Kyu-Bok Choi1, Seung-Jung Kim1
출판정보	2014; 2014(1):
키워드	신생혈관내막증식, 내피세포미세입자
초록	Background: Vascular access stenosis occurs frequently and predominantly as a result of neointimal hyperplasia (NH) formation due to smooth muscle cell (SMC) proliferation at the venous anastomosis. Effective treatment for NH in this setting is lacking. Endothelial microparticles (EMP) are closely associated with vascular dysfunction and atherosclerosis. We are to investigate the effects of EMP on proliferation of SMCs and NH formation. Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and stimulated by indoxyl sulfate (IS). The culture media of HUVECs were collected and ultracentrifugated at 100,000×g. The pellet was resuspended, incubated with fluorescent endothelial antibodies and analyzed by flow cytometry with comparing calibration beads. Human aortic smooth muscle cells (SMCs) were treated by these collected EMPs. The proliferarions of SMCs were measured by BrdU cell proliferation assay. Ex vivo culture experiments were also performed on porcine internal jugular veins with or without EMPs for 12 days, and compared with TGF-β treated venous tissues. Results: The CD31+CD42- EMPs were well produced by IS, the quantity of them was similar to the amount generated by positive control, TNF-α (10 ng/mL). EMPs stimulated the proliferation of cultured SMCs in a dose-dependent manner. In ex vivo culture, NH was significantly developed in EMP treated veins compared to controls with similar patterns stimulated by TGF-β. Conclusion: EMPs stimulate the proliferation of vascular SMCs and NH production in porcine vein. Further investigation is needed to demonstrate the role of EMPs on vascular access stenosis.
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