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논문분류	춘계학술대회 초록집
제목	The Effect of Bicarbonate Containing Peritoneal Dialysis Solution on Epithelial-to Mesenchymal Transition in Omentum-derived Mesothelial Cells
저자	Kyu-hyang Cho, Jun-young Do, Jun-hyeok Seo, Eun-woo Choi, Mun-ju Hwang, Soek-hui Kang,Jong-won Park, Kyung-woo Yoon, Yoon-mi Suk, Kei-lim Shin, Hyeon-ja Son, Soo-jeong Lee
출판정보	2014; 2014(1):
키워드	상피간엽전이, 복막투석액, 장막
초록	Background: The purpose of this study is to investigate the effect of bicarbonate containing PD (peritoneal dialysis) solution on EMT (epithelial-to mesenchymal transition) in omentum-derived mesothelial cells. Methods: Omentum-derived mesothelial cells from 20 nonuremic patients undergoing abdominal surgery were incubated with lactate-buffered standard PD solutions (L group; Dianeal® and Stay-safe®), bicarbonate/lactate buffered PD solutions (LB group; Physioneal® and Balance®) or bicarbonate buffered PD solution (B group; BicaVera®) diluted 1:1 with culture medium. E-cadherin was measured as standard mesothelial marker by quantitative RT-PCR (real time-polymerase chain reaction) analysis. Snail and alpha-SMA (alpha-smooth muscle actin) were also measured as fibroblast marker by quantitative RT-PCR analysis. The number of CD54 (cluster of differentiation 54) positive cells (indicative mesothelial cells) was counted among confluent omentum-derived mesothelial cells by FACS (fluorescence- activated cell sorter). Results: There were no significant differences in level of E-cadherin, snail and alpha-SMA among three groups. The number of CD54 positive cells among confluent omentum-derived mesothelial cells was B group, BL group and L group in order but the difference did not reach the statistical significance. Conclusions: Bicarbonate containing PD solution groups showed a trend of higher number of CD54 positive cells among confluent omentum-derived mesothelial cells in vitro but without significant statistical differences in this study. We need further studies to clarify the impact of bicarbonate containing peritoneal dialysis solution on EMT in mesothelial cells.
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