대한신장학회

논문분류	춘계학술대회 초록집
제목	Experimental Approach to Evaluate the Role of TNFSF13 in the Progression of IgA Nephropathy
저자	Seung Seok Han1, Seung Hee Yang2, Murim Choi3, Kyung Chul Moon4, Hang-Rae Kim5, Hajeong Lee1, Jung Pyo Lee1, Kwon Wook Joo1, Chun Soo Lim1, Shin-Wook Kang6, Yon Su Kim1, Dong Ki Kim1
출판정보	2015; 2015(1):
키워드	면역글로불린 A􀀁= 신병증, B 림프구, 예후
초록	Background: We had previously reported that tumor necrosis factor superfamily 13 (TNFSF13) is associated with both the susceptibility to and progression of IgA nephropathy. In this study, we further evaluated the role of TNFSF13 in the IgA nephropathy as in vitro study. Methods: We isolated B cells from IgA nephropathy patients (n=30) and healthy individuals (n=29), and stimulated the cells with/without TNFSF13. We evaluated the production and proportion of IgA and galactose-deficient IgA (GdIgA) by ELISA and flow cytometry. Additionally, we estimated the proportion of viable B cells by annexin V staining and examined whether glycosyltransferase expression is altered with TNFSF13 treatment by the realtime PCR and RNA sequencing. Furthermore, we compared the ectopic expression of TNFSF13 mRNA in renal tissues from healthy individuals with that in IgA nephropathy and lupus nephritis patients. Results: After 4 days of culture, the total IgA level was higher in TNFSF13-treated group than in the control group. IgA levels reduced upon treatment with receptor blockers (TACI-Fc or BCMA-Fc). Similar results were also obtained by flow cytometry and in the healthy individuals. However, unrelated or contrasting results were obtained for the effect of TNFSF13 treatment on GdIgA levels. ELISA revealed that the proportion of GdIgA was reduced in the TNFSF13-treated group; flow cytometry did not reveal any correlation between TNFSF13 and GdIgA+ B cell proportion. Additionally, TNFSF13 treatment did not alter the expression of glycosyltransferase mRNA. Annexin V staining revealed that the viability of B cells was significantly higher in the TNFSF13-treated group than the control group or in the group with receptor blockers. Tubular or glomerular expression of TNFSF13 did not differ between the healthy controls and IgA nephropathy patients; however, tubular expression was higher in lupus patients than in the other groups. Conclusions: TNFSF13 affects the outcome of IgA nephropathy by enhancing the survival of B cells rather than by increasing GdIgA production.
원문(PDF)	PDF 원문보기

간행물 검색

대한신장학회 회원관리 규정