대한신장학회

논문분류	춘계학술대회 초록집
제목	Genetic Profiling of miRNAs Reveals the Role of p53 and Wnt Signaling Pathway in Secondary Hyperparathyroidism
저자	Yu Ah Hong1, Jeong Yup Kim2, So Yeon Bae3, Mi Yeon Jung3, Sun Woo Kim3, Gang Jee Ko3, Jae Bok Lee4, Heui Jung Pyo1, Young Joo Kwon1
출판정보	2015; 2015(1):
키워드	부갑상선 기능항진증,miRNA,p53
초록	Background: MicroRNAs (miRNAs) are small non-coding, single-stranded RNAs that regulate messenger RNAs at the post-transcriptional level. They have been identified as mediators of various signaling pathway such as cell proliferation and growth of neoplasm. Secondary hyperparathyroidsm (SHPT) should be treated with surgery in some cases, however the exact mechanisms why the glands gains autonomy with poor response to medication remains unclear. We aim to investigate whether genetic profiling of miRNA of parathyroid gland in SHPT would be useful to predict the possiblity for surgical treatment. Methods: Patients were enrolled who needed to receive parathyroidectomy for SHPT irresponsive to medication. Parathyroid tissues for normal controls were accidentally obtained from patients receiving thyroid lobectomy. We performed a TaqMan low-density array-based RT-PCR miRNA profiling, and compared with expressions of 384 miRNA expression between patients and controls. Results: In parathyroid nodular hyperplasia, mean pre-operative intact parathyroid hormone (PTH) levels were 1781.2±864.2 pg/ml, and mean parathyroid volume was 4.5±2.70 cm3. Twelve miRNAs (miR-103, miR-1271, miR-1275, miR-1291, miR-148b, miR-18a, miR-193a-3p, miR-21, miR-301, miR-425-5p, miR-532, and miR-532-3p) were significantly up-regulated (>2 fold change), and miRNA-139-5p, which had anti-tumorogenic and anti-metastatic activity, was significantly down-regulated in parathyroid nodular hyperplasia compared to normal controls. The p53 expression was negatively correlated with miRNA-139-5p expression significantly in parathyroid nodular hyperplasia (r=-0.892, p=0.042). Non-phospho β-catenin expression in nucleus was significantly increased in parathyroid nodular hyperplasia compared to normal control groups. But, there were no significant associations with p21 and p27 expression. Conclusions: These results suggest that genetic profiling of miRNA in SHPT might be useful to predict surgical treatment of SHPT and miR-139-5p might have a role in the proliferation of parathyroid cell in SHPT through p53 pathway.
원문(PDF)	PDF 원문보기

간행물 검색

대한신장학회 회원관리 규정