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논문분류	춘계학술대회 초록집
제목	Comparative Proteomic Analysis of Rapamycin Versus Cyclosporine Combination Treatment in Mouse Podocyte
저자	Beom Seok Kim1, Yuri Cho1, HyoJung Lee1, Dong Jin Joo2, Kyu Ha Huh2, Myoung Soo Kim2, Yu Seun Kim2
출판정보	2015; 2015(1):
키워드	족세포,라파마이신,타크로리무스
초록	Background: The mechanism of proteinuria observed with rapamycin (RPM) use remains unclear. The conversion from calcineurin inhibitors (CNIs) to RPM in kidney transplant recipients has been associated with a higher incidence of proteinuria. In this study, we performed proteomic analysis to investigate the alteration of protein expression in mouse podocyte treated with RPM in comparison with RPM/CNI combination. Methods: Immotalized mouse podocytes were treated with 20 nM RPM or 20 nM RPM+1 μg/ml cyclosporine. Podocyte proteins were separated by two dimensional-polyacrylamide gel electrophoresis (PAGE) and identified by matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry and peptide fingerprinting. Selected proteins were analyzed by western blot assay. Results: We identified 36 differentially expressed proteins after isolated RPM or RPM/CNI combination treatment in cultured mouse podocytes. There are three distinct patterns of protein expression: 1. Potentiated down-regulation of proteins by RPM/CNI treatment compared with isolated RPM treatment (n=4); 2. Partial offset of down-regulation by RPM/CNI in comparison with RPM (n=25); 3. No difference in down-regulation between RPM and RPM/CNI (n=7) Conclusion: We found a significant interplay between RPM and CNI on the proteins expression in mouse podocyte. This might explain the higher incidence of proteinuria by RPM/CNI combination in clinical settings. Further study is required to elucidate the target protein associated with RPM induced proteinuria.
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