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논문분류	춘계학술대회 초록집
제목	Immune Modulatory Effect of Thalidomide and Dexamethasone Co-treatment on T Cell Subsets
저자	Beom Seok Kim1,2, Joon Ye Kim1, Eun Jee Kim1, Yuri Cho1, Dong Jin Joo1,3, Kyu Ha Huh1,3, Myoung Soo Kim1,3, Yu Seun Kim1,3
출판정보	2015; 2015(1):
키워드	T 세포,탈리도마이드,덱사메타손,면역 조절
초록	Background: Thalidomide (TM) is known to have anti-cancer and anti-inflammatory properties; however, its mechanism on T cells is still unclear. Previously we showed immune modulatory effect of TM on T cells, and corticosteroid potentiates therapeutic effect of TM on lupus nephritis model. Here we examined whether TM/corticosteroid co-treatment has synergistic immune modulatory role on T cells. Methods: Splenic naive T cells (Tnaives) from C57BL/6 mice were sort-purified and cultured for CD4+ T cell proliferation and regulatory T cells (Tregs) conversion with TM and/or dexamethasone (DX) treatment. Also T cell suppression assay was performed to evaluate the function of converted Tregs. All samples were analyzed by flow cytometry after stained with anti-mouse CD4, Foxp3, OX40 (CD134), or glucocorticoid-induced TNFR-related protein (GITR; CD357). Results: TM significantly decreased the proliferation of CD4+ T cells in dose-dependent manner (p<.01) and low dose DX co-treatment further decreased the proliferation synergistically (p<.03). In contrast, TM/DX co-treatment ameliorated the inhibitory effect and function of isolated DX on Treg conversion (p<.04). Furthermore DX treatment impaired the functions of converted Treg, which was recovered by TM/DX co-treatment. Also, Reduced GITR and OX40 expressions by DX treatment were ameliorated by TM/DX co-treatment (GITR; p<.01, OX40; p<.04). Conclusion: Considering the selective effect of TM on different T cell subsets, TM may have an immune modulatory role and DX co-treatment could further enhance the effect partially by the change of GITR and OX40 expression on Tregs. Further study is required to elucidate the underlying link between corticosteroid and thalidomide effect on T cells.
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