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논문분류	춘계학술대회 초록집
제목	Fecal Transplantation from Uremic Mice Aggravates Kidney Fibrosis
저자	Sungyoon Lim* 1, Hee Young Lee1, Yoon Suk Go1, Jihyun Yang1, Young Joo Na1, Sang-Kyung Jo1, Won-Yong Cho1
출판정보	2016; 2016(1):
키워드	fecal transplantation, Kidney Fibrosis, kidney-gut crosstalk
초록	Background: Emerging evidence showed the important role of kidney-gut crosstalk in diverse pathological processes. Alterations in intestinal barrier or microbiota has been demonstrated in chronic kidney disease (CKD) and thought to be associated with increased cardiovascular risks or progression of CKD. The purpose of this study was to investigate the effect of gut microbiota in the animal model of kidney fibrosis with using fecal transplantation. Methods: Unilateral ischemia/reperfusion injury (IRI) for 45min was performed in C57/BL6 mice for fibrosis model. Supernatants of centrifuged feces from 5/6 nephrectomized mice (8wks) or age matched control mice were administered 3 times per wk for 3 wks after gut decontamination and degree of fibrosis was compared (CKD feces vs control feces). Results: Compared to mice with fecal transplantation from control mice, mice who were transplanted with CKD feces showed aggravated fibrosis at 2 wks after unilateral ischemia in the analysis of the Masson’s trichrome staining and Western blot for type 4 collagen. Ex vivo analysis of immune cells showed increased number of mature CD80+ CD11c+ cells in mesenteric lymph node whereas percentage of splenic Tregs increased in mice transplanted from uremic mice. Immune cells from these mice showed significantly suppressed cytokine release upon LPS stimulation compared mice with WTfeces. Conclusion: This study showed the possible effect of kidney-gut crosstalk on the progression of CKD and this effect is thought to be partially mediated by immune modulatory effect.
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