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논문분류	춘계학술대회 초록집
제목	Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy
저자	imic Hypertensive Nephropathy
출판정보	2016; 2016(1):
키워드	glomerulus-on-a-chip, hypertensive nephropathy, mechanic force, perfusion
초록	Background: To develop a microfluidic rapid evaluating of multi-inductors device to reproduce the structure and function of glomeruli. And studying the effect of glomerular hemodynamic on glometular filtration function under hypertension by microfluidic devise. Methods: This device consists of PDMS and polycarbonate membrane. Glomerular endothelial cell (MGEC) and podocyte (MPC-5) were co-cultured on the chip. MGEC cells and MPC-5 cells of each culiture unit were incubated under flow rate of 5μl/min，10μl/min，15μl/min medium. After 0h, 6h, 12h and 24h, the function of GFB were detected by liuorescence microplate, the expression of F-actin, CD-31, vWF of MGEC cells and F-actin, synaptopodin, nephrin, podocin of MPC-5 cells weredetected by immunofluorescent staining.The cell apoptosis was assessed by DAPI staining. Results: Compared with traditional cell-culture, MGEC cells and MPC-5 cells grew well and morphological features showed no significant change cell apoptosis.When MGEC cells and MPC-5 cells were treated with medium under 20μl/min, the function of GFB was promoted and the distribution of F-actin was changed. When MGEC cells and MPC-5 cells were treated with medium under flow rate of 10μl/min and 15μl/min.The function of GFB was impaired and the distribution of F-actin was changed obviously. CD-31 decreased and vWF increased progressively over flow rate increasing and time extend. Nephrin and podocin, markers of MPC-5 cells, deceased progressively over flow rate increasing and time(P<0.05). The cell apoptosis increased under 15μl/min for 24h（P<0.05） Conclusion: This work provides an reappearance of the hypertransfusion, high filtration and high transmembrane pressure in glomerular capillary when hypertension leads to renal blood flow decompensation by adjusting the fluid infusion speed on glomerular microfluidic device which confirmed directly that the abnormal hemodynamic factors can degrade GFB function by injuring the skelemin of endothelial cells and podocytes and ligandin between cells.
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