대한신장학회

논문분류	춘계학술대회 초록집
제목	Circulating cell-free genomic DNA, mitochondrial DNA and pentraxin-3 in the patients on hemodialysis and medical staffs during isolation by Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Korea
저자	Yang gyun KIM1, Haena MOON1, Da-wun JEONG1, *Sang-ho LEE1, Se-yun KIM1, Yu-ho LEE1, Kipyo KIM1, Ju young MOON1, Young-ki LEE2
출판정보	2017; 2017(1):
키워드	MERS-CoV, circulating cell-free genomic DNA, circulating cell-free mitochondria DNA, pentraxin-3
초록	Objectives : The plasma level of circulating cell free genomic DNA (ccf-gDNA), mitochondrial DNA (ccf-mtDNA) and pentraxin-3 (PTX-3) is known to be elevated under inflammatory or stress condition. At the outbreak of the Middle East Respiratory syndrome coronavirus (MERS-CoV) in 2015, hemodialysis (HD) patients and medical staffs in our center were exposed to one patient infected by MERS-CoV and have been isolated during 2 weeks. This study was performed to investigate clinical meaning of ccf-gDNA, ccf-mtDNA and PTX-3 in HD patients and medical staffs during isolating period. Methods : The plasma of 85 HD patients at predialysis and 12 medical staffs at morning time was collected at 2 week, 4 week, and 16 week after isolation. Real-time PCR for ccf-gDNA and ccf-mtDNA and ELISA for PTX-3 were done. General characteristics for HD patients were also analyzed. Results : The log level of ccf-gDNA was significantly decreased from 2 to 4 week, and from 4 to 16 week in HD patients. However medical staffs showed abrupt decline of ccf-gDNA from 2 to 4 week then, there was no change between 4 and 16 week. The level of ccf-gDNA at 4 week was significantly higher in HD patients than that of medical staffs. The level of ccf-mtDNA was not changed between 2 and 4 week, then it was significantly decreased from 4 to 16 week in HD patients. Although the value of ccf-mtDNA was similar in HD patients and medical staff, ccf-mtDNA of medical staffs was significantly declined from 2 to 4 week, and from 4 to 16 week. During isolation period, PTX-3 was elevated only in HD patients. The value of PTX-3 was highest at 2 week and significantly decreased at 4 week, then became similar with that of medical staffs at 16 week. The patients receiving adequate care of anemia (Hb≥ 10g/dL) and dialysis (Kt/V≥ 1.2) were 59.1% and 95.3% at 2 week after isolation, then it was elevated to 87.8% and 100% at 16 week. Conclusions : Elevated ccf-gDNA and ccf-mtDNA in HD patients and medical staffs reflects extremely physical and emotional stress during isolation. However, the level of cfDNA and mtDNA was recovered slowly and PTX-3 was only increased in HD patients. It was suggested that the inflammatory response under stress was intensified in HD patients and poor management during isolating period could influence these findings.
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