대한신장학회

Skip Navigation: Skip to contents

KOR
ENG
전체메뉴보기

간행물 검색

현재 페이지 경로

HOME
간행물
간행물 검색

논문분류	춘계학술대회 초록집
제목	Loss of KLF15 promotes chronic podocyte injury
저자	Seung seok HAN1, Hajeong LEE1, Jung pyo LEE1, Ran-hui CHA2, Dong ki KIM1, Yon su KIM1, *Seung hee YANG2
출판정보	2017; 2017(1):
키워드	chronic kidney disease; cyclosporine; Krüppel-like factor 15; podocyte
초록	Objectives : Krüppel-like factor 15 (KLF15), kidney-enriched transcription factor, is known to participate in the differentiation of podocyte. However, the implication of KLF15 in chronic podocyte injury remains unresolved, particularly in the relationship with the infiltrated macrophage. Methods : 5/6 nephrectomized and C-C chemokine receptor type 5 (CCR5)-/- mouse models were used to determine chronic podocyte injury and explore exclusive role of M1 macrophage in the KLF15 expression, respectively. Human primary podocytes were flow-cytometically isolated and cultured to emulate the injury process in the in vitro system. Biopsied kidney tissues were obtained from the patients with diabetic nephropathy (n=21) and primary membranous nephropathy (n=26) to elucidate the relationship between glomerular KLF15 expression and subsequent outcomes. Results : When 5/6 nephrectomy was predisposed to progressive kidney damage, the fibrosis markers increased, but the KLF15 expressions decreased in the site of podocytes. The KLF15 expression was further reduced by the infiltration of M1 macrophage using CCR5-/- mice, which resulted in more intense fibrosis [Figure]. We also observed corresponding trends in human primary podocytes, such as increase in fibrosis markers and decrease in KLF15 production after 2-day treatment of TGF-β. These trends were reversed when cyclosporine or tacrolimus were treated in cultured podocytes. When patients were categorized based on the KLF15 expression levels in kidney tissues, the low expression groups suffered worse kidney events such as end-stage renal disease and non-remission of disease. Conclusions : Low KLF15 expression in podocytes is associated with chronic kidney injury.
원문(PDF)	PDF 원문보기

목록

위로가기