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논문분류	춘계학술대회 초록집
제목	Inhibition of CXCR3 expression through blockade of STAT3 alpha signaling down-regulate inflammation of renal ischemia-reperfusion injury
저자	Jae Yoon Park1, Kyung Don Yoo2, Jae Wook Lee5, Sung Joon Shin1, Kyung Soo Kim1, Yon Su Kim3, Seung Hee Yang4
출판정보	2018; 2018(1):
키워드	STAT3 alpha \| CXCR3 \| ischemia-reperfusion injury \| acute kidney injury
초록	Objectives: Signal transducer and activator of transcription 3 (STAT3) is the main mediator of interleukin 6 (IL-6)–type cytokine signaling. Although it exists in two isoforms: the full-length STAT3α and the truncated STAT3β, their role in acute kidney injury is not clarified. We investigated their relative function through inhibiting STAT3α in ischemia-reperfusion (IR)-induced renal inflammation. Methods: IR injury was induced in B6 wild type mice. Stattic ( nonpeptide small molecular inhibitor of STAT3 activation and dimerization) was treated 3 hours prior to IR injury. We quantified intrarenal cytokine expression using real-time PCR and performed FACS analysis. We cultured human tubular epithelial cells (TECs) in hypoxic condition and evaluated the effect of Stattic treatment. We detected the isoforms of phosphorylated STAT3 using western blot analaysis. Results: IR injury produced more severe tubular damage in control group than in Stattic-treated mice (serum creatinine, 2.2±0.1 versus 1.6±0.1 mg/dL, p<0.05). Although inflammatory cytokines/chemokines, such as IL-6, total STAT3, STAT3α, CXCR3, IL-10 and TGF-β were increased by IR injury in control group, they were attenuated in Stattic-treated mice. Apoptosis of TECs and infiltration of mononuclear cells and macrophages were decreased and the expression of STAT3α as well as total STAT3 was reduced in Stattic-treated mice. These findings were supported by in-vitro study with human TECs. Whereas the level of pSTAT3α was elevated in hypoxia-conditioned TECs and it was decreased in Stattic-treated cells, the level of pSTAT3β was not changed in both cell groups. The expression of CXCR3 was decreased in accordance to the STAT3α decrease and the supernatant levels of IL-6 and IL-8 were decreased in Stattic-treated cells. Conclusions: We demonstrated that activation of STAT3 is associated with progression of IR injury and the α-isoform may contribute as major player. These mechanisms of STAT3/CXCR3 signaling according to each isoforms suggest a novel strategy for management of AKI with STAT3 inhibitor.
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