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논문분류	춘계학술대회 초록집
제목	The antioxidant nanoceriaameliorates diabetic podocyte injury by decreasing high glucose-induced oxidative stress
저자	Se Hee Yoon1, Sung Kwon Cho1, Won Min Hang1, Jaegu Kang2, Seong-Lan Yu2, San Eun Hong3, Kuk Ro Yoon3, Sung-Ro Yun1
출판정보	2018; 2018(1):
키워드	diabetes \| podocyte \| nanoceria \| antioxidant \| reactive oxygen species
초록	Objectives: Podocyte injury, a major contributor to the pathogenesis of diabetic nephropathy, is caused at least in part by the excessive generation of reactive oxygen species. The use of antioxidants can benefit the control and prevention of diabetes side effects. Cerum oxide nanoparticles (nanoceria) exhibit superoxide dismutase and catalase mimetic activities.We investigated the effect of ceria nanoparticles in cultures of high glucose exposed human podocytes. Methods: In this experimental study, we divided human podocyte into theses groups: i. cell treated with 5mM D- glucose (control), ii. cells treated with20mM D-mannitol+5mMD glucose (osmotic control), iii. Cells treated with 25mM D glucose (high glucose) and iv. cell treated with 25mM D-glucose +nanoceria. MTT assay, ROS formation (ampelx red assay, DHE stain and DCF-DA), fibrosis marker genes (TGF-β, collagen IV, α-SMA and fibronectin) and apoptosis marker (caspase 4) were measured and analyzed statistically. Results: High glucose (25 mM) treatment increased oxidative stress and fibrosis markers in human podocytes. Nanoceria at a concentration of 10 ug/ml significantly decreased the high glucose induced cytotoxicity, ROS formation and fibrosis markers. Conclusions: The antioxidant of nanoceria particles have the potential as a therapeutic medicine for preventing ROS-related hyperglycemia oxidative damage
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