대한신장학회

논문분류	춘계학술대회 초록집
제목	Treatment of 6-bromo-indirubin-3’-oxime (6-BIO) attenuates inflammation of proximal tubular epithelial cells through suppression of adhesion molecules and monocyte chemoattractant protein-1 (MCP-1) in LPS-stimulated mononuclear cells
저자	Jung Sun Park, Hoon-In Choi, Dong-Hyun Kim, Eun Hui Bae, Seong Kwon Ma, Soo Wan Kim
출판정보	2018; 2018(1):
키워드	6-BIO \| MCP-1 \| adhesion molecule \| inflammation \| HK-2 cells
초록	Objectives: Sepsis is a clinical problem which causes an acute kidney injury (AKI) and a high risk of developing chronic kidney disease (CKD). The aim of this study was to investigate whether there was a cross-talk between tubular epithelial cells and mononuclear cells in the lipopolysaccharide induced inflammation, and whether 6-BIO, a glycogen synthase kinase-3β inhibitor, attenuated inflammation in human proximal tubular epithelial cells co-cultured lipopolysaccharide stimulated mononuclear cells. Methods: We evaluated the inflammatory response such as cytokine, chemokines, and interaction adhesion molecules in human proximal tubular epithelial (HK-2) cells co-cultured with LPS-stimulated mononuclear cells. The effects of 6-BIO on LPS-induced cytokine and chemokine, and adhesion molecules were determined using co-culture of human renal proximal tubular epithelial (HK-2) cells and human mononuclear (U937) cells. The effects of LPS and 6-BIO on cell viability were determined using EZ-CyTox assays. The protein and mRNA expression of MCP-1, cadherin, and selectin was determined by semiquantitative immunoblotting and RT-PCR. Intracellular staining of TNF-α, INF-γ, IL-6 was determined by using flow cytometry and confocal laser microscopy. Results: HK-2 cells co-cultured with LPS-stimulated mononuclear cells showed increased expression of inflammatory cytokine such as MCP-1, INF-γ, TNF-α, and IL-6 and adhesion molecule such as E-selectin, L-selectin, ICAM-1, and VCAM-1. Pre-treatment of 6-BIO in LPS-stimulated mononuclear cells and co-cultured with HK-2 cells revealed significantly attenuated the expression of cytokine, chemokine, and adhesion molecules compared with in HK-cells co-cultured with LPS-stimulated mononuclear cells without 6-BIO pretreatment. Conclusions: Glycogen synthase kinase-3β inhibitor, 6-BIO attenuated inflammation in human proximal tubular epithelial cells through suppressing the activation of cytokine, chemokines, and interaction adhesion molecules in LPS stimulated mononuclear cells.
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