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논문분류	춘계학술대회 초록집
제목	Effect of graphene-based nanomaterials treatment in kidney fibrosis
저자	Lilin Li, Joo Hee Kim, Seung Hee Yang, Joo Hong Joun, Jung Nam An, Jeong Hwan Lee, Young Wook Choi, Byung Hee Hong, Jung Pyo Lee
출판정보	2019; 2019(1):
키워드	graphene quantum dots (GQDs) \| chronic kidney disease \| unilateral ureteral obstruction \| unilateral ischemia-reperfusion \| TGF-β1/Smad signaling pathway
초록	Graphene derivatives - Graphene quantum dots (GQDs) have drawn much attention for its biomedical applications, such as bioimaging, drug delivery, gene delivery, and tissue engineering. At present, the role of GQDs in fibrotic diseases remains unclear. Fibrosis is a common pathological feature in most kinds of chronic kidney disease (CKD). Therefore, in this study, we aim to further determine whether GQDs can attenuate renal fibrosis by reducing tubulointerstitial injury in the CKD and in the progression of acute kidney injury to kidney fibrosis. Unilateral ureteral obstruction (UUO) and unilateral ischemia-reperfusion injury (UIRI) were induced in 7- to 8-wk-old male wild-type C57BL6 mice. GQDs were injected in both kidney fibrosis models through the tail vein. Histopathological examination was performed on the kidneys using Masson's trichrome stain, Sirius red stain, PAS stain and immunohistochemistry. TGF-β1 was used in vitro experiments to induced human kidney primary tubule epithelial cells. Real-time PCR and western blot analysis were used to detect the expressions of related molecules. Histopathological examination showed that GQDs treatment significantly attenuated interstitial fibrosis in the both kidney disease models. GQDs administration significantly reduced the expression of α-smooth muscle actin, collagen I, and fibronectin, however, it increased the expression of E-cadherin. In addition, GQDs also significantly reduced TGF-β1 and p-Smad2/3, on the other hand increased the expression level of Smad7. GQDs may protect against kidney fibrosis by inhibition of TGF-β1/Smad signaling pathway.
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