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논문분류	춘계학술대회 초록집
제목	Protective effect of biofabricated Prosopis cineraria silver nanoparticles against renal carcinoma via knock down oxidative stress and inflammation by regulating NF-κB pathway
저자	Ekta Yadav, Amita Verma
출판정보	2019; 2019(1):
키워드	renal carcinoma
초록	Renal cell carcinoma is considered as the most frequent and lethal cancer of urinary system. Since Prosopis cineraria (PC) leaves are rich in polyphenolic compounds and their silver nanohybrids potentially inhibit the human breast cancer cells (MCF-7). Considering the previous facts the current study was designed to bio-fabricate, characterize and to evaluate the protective efficacy of PC extract mediated silver nanoparticles (AgPCNPs) against renal carcinoma in Wistar rats. AgPCNPs were synthesized by using co-precipitation method and characterized by various techniques. Renal cancer in animals was initiated by injecting N-nitrosodiethylamine (DEN, 200 mg/kg, i.p.) for single time and further promoted by using ferric nitrilotriacetate (FeNTA, 9 mg Fe/kg, i.p.) treatment twice weekly till next 16 consecutive weeks. Simultaneously, for 16 weeks animals were administered with AgPCNPs at two dose levels (10 and 20 mg/kg p.o.). To assess the underlying molecular mechanism concerned with the anticarcinogenic activity of AgPCNPs, its effect on tumour marker enzymes (G6PD), serum marker enzymes (Creatinine and BUN) and enzymatic antioxidant levels (SOD, CAT and GPx) along with proinflammatory cytokines and mediators (IL-6, IL-1b, TNF-α, and NF-κB) was observed. Histological analysis was also performed with renal tissue. Different characterization techniques confirmed the formation of spherical crystalline nanoparticles with size range of 50-80 nm showed UV-Visible absorbance peak at 420 nm. DEN-FeNTA enhanced the serum toxicity marker, tumour marker enzyme as well proinflammatory mediators and cytokines whereas it reduced the antioxidant enzyme activity. AgPCNPs pretreated rats significantly restored (p<0.05) DEN-FeNTA induced damaging effects in a dose dependant manner as compared to DEN-FeNTP alone group. Histological characterization also supported the protective effect of AgPCNPs by potentially recovering the renal cellular structure in DEN-FeNTA induced cancer rats. AgPCNPs possess strong efficacy to prevent DEN-FeNTA induced renal cancer in rodents through regulation of oxidative stress and inflammation via NF-κB pathway.
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