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논문분류	춘계학술대회 초록집
제목	Three-dimensional visualization of renal resident mononuclear cells with clearing in murine model of acute kidney injury
저자	Ju-Young Moon, Dong-Jin Kim, Su-Woong Jeong, Yang-Gyun Kim, Sang-Ho Lee, Seung-Hae Kim
출판정보	2019; 2019(1):
키워드	Clearing \| renal resident monocuclear cell \| acute kidney injury
초록	Traditional histologic methods are limited in their ability to detect pathologic changes in acute kidney injury. Recently, a multiphoton microscopy (MPM) with optical sectioning is an emerging method that provides three-dimensional visualization in detail. However, few studies applied this new technique to a murine model of acute kidney injury. To induce acute kidney injury, CD11c-yellow fluorescent protein (YFP) mice received lipopolysaccharide (5µg/g) intraperitoneally, whereas a control group of CD11c-YFP mice did an equal volume of 0.9% saline. By applying the clearing with CLARITY, we compared CD11c-YFP cells within one millimeter-thick renal sections, which reflect resident renal mononuclear cells, between the murine model of acute kidney injury and controls. Mice that LPS was administered showed higher levels of serum blood urea nitrogen and creatinine than the control group ( 0.1 vs 0.6 mg/dL; p < 0.05). The main distribution of CD11c-YFP cells was located in the cortex and especially the tubulointerstitial area. The imaging of MPM demonstrated that number CD11c-YFP cells were significantly more densely interspersed within millimeter-thick tissue of acute kidney injury model compared with that of control (302 ± 16 vs 390 ± 21 cells; p < 0.05 ). Furthermore, it revealed that volume of CD11c-YFP cells more than 200 µm3 were more frequently observed in mice with acute kidney injury than the control group ( 381 ± 50 vs 421 ± 100; p < 0.05). MPM combined with optical clearing clearly provides the spatial distribution of target cells and its accurate counting within millimeter-thick tissue of murine model of acute kidney injury.
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