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논문분류	춘계학술대회 초록집
제목	Clinical significance of CD8+ T cell subset analysis for the prediction of acute allograft rejection in kidney transplant recipients
저자	Kyoung-Woon Kim, Jung-Woo Seo, Eun Jeong Ko, Bo-Mi Kim, Jang-Hee Cho, Chan-Duck Kim, Chul Woo Yang, Sang-Ho Lee, Byung Ha Chung
출판정보	2019; 2019(1):
키워드	Acute rejection \| CCR7+CD8+ T cells \| effector T cells \| kidney transplantation
초록	In this study, we performed multi-color flowcytometry to analyze the CD8+T cell subsets in KTRs with acute rejection in comparison with KTRs with different clinical status. At first, we included 121 KTRs from three transplant centers and we isolated PBMC at the time of allograft biopsy. They were divided into 3 groups according to the pathologic diagnosis; Normal biopsy control (NC) (n=32), Acute rejection (AR) (n=50), long term graft survival (LTGS) (n=39). We performed multi-color flowcytometry analysis using following platform. We also did microarray analysis to investigate the significant changes in gene expression associated with cell types using SAM analysis. The percentage of CCR7+CD8+ T cells showed significant decrease in the AR group compared to NC group or LTGS group (P<0.05 for each). In contrast, the percentage of CD28nullCD57+ T cell and CCR7-CD45RA+/CD8+ T cells and showed increase in AR group in comparison with other groups. Both effector CD8+ T cells showed significant negative correlation to CCR7+CD8+ T cells in an ex vivo and also in an vitro conditions. In microarray analysis from blood of KTRs, we identified significant genes associated with CCR7-CD45RA+/CD8+ T and CD28nullCD57+/CD8+ T cells by SAM analysis. Moreover, some of significant genes associated with CCR7-CD45RA+/CD8+ T cells and CD28nullCD57+/CD8+ T cells showed negative correlation to expressed genes in CCR7+CD8+ T cells. ROC analysis showed that the ratio between CCR7+CD8+ T cells and CD28nullCD57+ CD8+ T cells showed best prediction for AR in the whole clinical cohort (AUC=0.800, P<0.01). This study suggests that combined monitoring of the ratio between CCR7+ and CD28nullCD57+ T in CD8+ T gaiting may be useful for the prediction of AR. Validation of this result in the prospective cohort may be required.
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