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논문분류	춘계학술대회 초록집
제목	Kidney decellularized extracellular matrix hydrogels enhance vascularization and maturation of human iPSC-derived kidney organoids
저자	Sun Ah Nam, Jae Yeon Lee, Yoo-mi Choi, Jae Yun Kim, Jin Won Kim, Eunjeong Seo, Dong-Woo Cho, Yong Kyun Kim
출판정보	2020; 2020(1):
키워드	organoid \| KdECM
초록	Background: The human pluripotent stem cells (hPSCs)-derived kidney organoids contain segmented structures with podocytes, proximal tubules, and distal tubules in nephron-like arrangements. However, limited vascularization and immaturity of nephron-like structures have been still remain to overcome. The extracellular matrix (ECM) hydrogel can provide mechanical support and biochemical microenvironment for cell growth and differentiation. Here we report a culture system of hPSCs-derived kidney organoids with kidney decellularized extracellular matrix (dECM) hydrogel Method: Kidney dECM hydrogel was prepared from Porcine kidney tissue. Human iPSCs were differentiated into kidney organoids, which were culture with kidney dECM hydrogel for 7days. Kidney organoids were also transplanted with kidney dECM in renal subcapsular space of immunodeficient mice Results: Kidney dECM hydrogel enhanced vascularization of kidney organoids with upregulated vascular endothelial growth factor expression. Kidney dECM hydrogel also upregulation of gene expression for podocytes and tubular epithelial cell maturation. Transplantation of kidney organoids with kidney dECM accelerated recruitment of endothelial cells from host mouse kidney and maintained vascular integrity. In transplanted kidney organoids with kidney dECM, the slit diaphragm-like structures were more organized compared to those in without kidney dECM Conclusion: Our data suggest that microenvironment provided from kidney dECM hydrogels promote vascularization and maturation of human iPSC-derived kidney organoids, which can be applied for the development of vascularized kidney on a chips and the regenerative therapeutic option
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