대한신장학회

논문분류	춘계학술대회 초록집
제목	Scintigraphic Evaluation of Renoprotective Effects of Coenzyme Q10 in a Rat Renal Ischemia-Reperfusion Injury
저자	Aylin AKBULUT, Bugra Keseroglu, Gokhan Koca, Hatice Surer, Elmas Ogus, Nihat Yumusak, Cem Nedim Yuceturk, Berat Cem Ozgur, Meliha Korkmaz
출판정보	2019; 2019(1):
키워드	ischemia-reperfusion injury \| coenzyme Q10 \| MAG3 scintigraphy \| anti-apoptotic \| anti-peroxidative
초록	Renal ischemia-reperfusion injury (RIRI) may occur secondary to various reasons and may lead to acute or chronic renal failure. Coenzyme Q10 (CoQ10) is a well-known anti-oxidant that has been assessed in a variety of diseases due to oxidative stress and decreased antioxidant capacity. Still, the effects CoQ10 against RIRI have not been evaluated. Our aim was to evaluate the protective effects of CoQ10 to renal ischemia-reperfusion by biochemical, immunohistochemical and scintigraphic findings. 30 Wistar albino rats were randomly separated into three groups of 10; Group Sham; Group ischemia-reperfusion (IR) underwent renal ischemia by left renal pedicle clamping, and Group CoQ10+IR received CoQ10 and underwent IR. 24 hours later after the reperfusion, scintigraphy was performed in all groups before sacrification. To demonstrate the effects of RIRI, serum urea and creatinine levels and tissue levels of malondialdehyde (MDA), total sulphidrile (SH), total nitrite, nitrate and myeloperoxidase (MPO) markers of oxidative stress were evaluated. Both kidneys were subjected to histopathological evaluation and besides; to confirm RIRI induced immunohistochemical aspects of apoptosis TUNEL assay method and activated caspase-3 were assessed. The tissue oxidative stress levels, histopathologic changes, apoptosis scores and quantitative scintigraphic parameters were significantly higher in Group IR compared to Group Sham. Though tissue oxidative stress levels and histopathologic changes were not significant the quantitative scintigraphic parameters of the contralateral non-ischemic kidney of Group IR were significantly increased. Compared to Group IR, Group CoQ10+IR presented decreased tissue oxidative stress levels; decreased scores of histopathology and apoptosis, and decreased quantitative scintigraphic parameters with increased SRF in ischemic kidney. Our results suggest that other than its anti-oxidant properties, CoQ10 shows anti-peroxidative, anti-apoptotic and anti-inflammatory potential in protecting the renal functioning of the ischemic kidney. Furthermore our results show that renal scintigraphy is a feasible method to detect early changes in renal functioning after RIRI.
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